Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Hypoglycemic, analgesic and anti-inflammatory effects of Pulicaria incisa (Lam.) DC extracts in animal models

Djamila Sahar¹ , Somia Saad^1,2, Saida Ouafi¹

¹University of Sciences and Technology Houari Boumediene, Faculty of Biological Sciences, Research Laboratory on Arid Zones (LRZA), BP n° 32 El-Alia, Bab Ezzouar 16111, Algiers; ²Scientific and Technical Research Centre on Arid Regions (CRSTRA), Bioresources Division, Biskra, Algeria.

For correspondence:- Djamila Sahar Email: sahar.djamila@gmail.com

Accepted: 1 March 2022 Published: 31 March 2022

Citation: Sahar D, Saad S, Ouafi S. Hypoglycemic, analgesic and anti-inflammatory effects of Pulicaria incisa (Lam.) DC extracts in animal models. Trop J Pharm Res 2022; 21(3):589-596 doi: 10.4314/tjpr.v21i3.19

© 2022 The authors.
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Abstract

Purpose: To investigate acute toxicity, hypoglycaemic, analgesic and anti-inflammatory activities of Pulicaria incisa (Lam.) DC.

Methods: Acute toxicity analysis was assessed by adopting the OECD - 423 Guidelines. The hypoglycemic effect of P. incisa infusion was investigated utilizing Oral Glucose Tolerance Test (OGTT) on orally glucose-induced hyperglycemic rats. The analgesic effect was investigated using the writhing test model, and anti-inflammatory activity was ascertained by carrageenan-induced paw oedema method.

Results: The results showed that half-maximal lethal dose (LD₅₀) values in mice were higher than 14 g/kg. The results of OGTT showed that the plant infusion produced a significant (p < 0.05) reduction in the glucose level in the blood post oral glucose administration. Treatments with diethyl ether, n-butanol and free aglycone extracts at 400 mg/kg significantly reduced (p < 0.05) the abdominal cramps by 82.45, 74.36 and 65.35, % respectively. Diethyl ether extract also showed higher inflammatory inhibition (67 %) than standard (54 %). As predicted, these extracts were less effective against pain and acute inflammation at 800 mg/kg. The HPLC profile demonstrated the existence of phenolic acids, flavones and C-glycosides.

Conclusion: The findings show that P. incisa has promising hypoglycemic, analgesic and anti-inflammatory effects, which confirms its folkloric medicinal use in Algeria.

Keywords: Acute toxicity, Biological activities, Glucose level, HPLC, Pulicaria incisa